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António Machado
Researcher

My research focuses on the study of microbial biofilms and the development of alternative strategies for the control of multidrug-resistant microorganisms. I aim to understand how microbial communities organize themselves, interact with one another, and respond to the host, the environment, and different antimicrobial pressures, using molecular, microbiological, and imaging approaches, including PCR/qPCR, next-generation sequencing, fluorescence/confocal microscopy, and electron microscopy.     

An important component of my work is the evaluation of alternative antibiofilm and antimicrobial approaches, including bacteriophages, natural products, honey, plant extracts, probiotic-derived metabolites, and other bioactive compounds, either alone or in combination with conventional antimicrobials, with particular emphasis on multidrug-resistant pathogens.

I integrate these studies within a One Health framework, linking human, animal, and environmental health. My research addresses biofilm-forming microorganisms of clinical, food, aquaculture, and environmental relevance, including antimicrobial resistance in food chains and aquatic ecosystems. The ultimate goal is to contribute to more effective, sustainable, and applicable prevention and control strategies in Public Health, food safety, and environmental protection, promoting translational and interdisciplinary research

Publications

Cangui-Panchi, S.P., Cangui-Panchi, D.S., Palacios, V.E., Becerra, E., Santamaría, A.L., Muñoz, D., et al. (2026). Integrated Phenotypic, Proteomic (MALDI-TOF MS), and Genomic (WGS) Investigation of a Prolonged Hospital Outbreak of Pseudomonas aeruginosa with High Biofilm-Forming Capacity. Antibiotics, 15, 257.
Gomes, E., Mesquita, T.G., Serra, P., Araújo, D., Almeida, C., Machado, A., et al. (2026). Antimicrobial Resistance in the Food Chain: Bridging Knowledge Gaps for Effective Detection and Control. Antibiotics, 15, 262.
Gualle, A., Orejuela-Escobar, L.M., Celi, D., Nolivos-Rodriguez, K., Aguilera-Pesantes, D., Vargas, D., et al. (2026). Screening of antioxidant activity, antimicrobial properties, and chemical profiles of 31 plant species against Vibrio and Pseudomonas in the Ecuadorian shrimp industry. Journal of the World Aquaculture Society, 57.
López, L., Cangui, P., Guilcazo, D., Machado, A., Blount, Z.D. & Trueba, G.A. (2026). Fitness costs and persistence of plasmid-mediated cephalosporin resistance in Escherichia coli: an integrative review. Frontiers in Microbiology, 17, 1783087.
Vizuete, K.S., Cabascango, D.G., García, T.X.I., Machado, A.J.T., Pilaquinga, F., Fernandez, L.M., et al. (2026). Silver nanoparticles as antimicrobials: A comparative analysis of green and traditional chemistry synthesis methods. PLoS ONE, 21.